How To Clean Dog Urine From Tile Grout. With a sponge or cloth, swipe it around the surface until the grout lines are full. What the vinegar does is neutralize the dog urine and do away with the odor.

Prepare a mixture of warm water and vinegar and make sure that you put the same amount of both in a bowl. Mix the clear vinegar with water in a bucket, using 1 part vinegar to 10 parts water, for stains on sealed grout. Start by soaking up the puddle of urine with paper towels.